The present paper reports the photoluminescence (PL) and thermoluminescence (TL) studies of natural Bhutanese stone (Biotite) collected from Bhutan. The sample was Bhutanese stone irradiated with Co60 gamma source giving a dose from 0.5 kGy to 2 kGy. The heating rate used for TL measurements was 6.7° C/s. The heating rate used for TL measurements was 6.7° C/s. The samples shows good TL peaks, the corresponding activation energy (E) values are calculated. Structural characteristics were showed by XRD spectrum of the sample. The value of trap depth of Bhutanese stone was evaluated by different methods. Also the annealing quenching effect was studied for gamma exposure of the sample.